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Detection of Virulence Factors and Antibiotic Resistance Pattern of Clinical and Intensive Care Unit Environmental Isolates of Pseudomonas aeruginosa

Author(s):

Omid Zarei, Hassan Mahmoudi, Ali Mohammadi Bardbari, Pezhman Karami and Mohammad Yousef Alikhani*   Pages 1 - 5 ( 5 )

Abstract:


Background: Pseudomonas aeruginosa is a gram-negative non-glucose fermenting aerobic bacterium and an opportunistic pathogen in humans and animals. The present study was carried out in order to investigate the distribution of virulence factors and antibiotic resistance properties of P. aeruginosa isolated from patients and intensive care unit (ICU) environment.

Material and Methods: A total of 116 P. aeruginosa isolated from patients and ICU environment were collected from Besat hospital in Hamadan, west of Iran. P. aeruginosa isolates were analyzed based on the presence of the virulence factors encoding genes included exo A, exo S, exo U, and alg D using polymerase chain reaction (PCR). Antimicrobial susceptibility test was performed using a disk diffusion method.

Results: The results showed the prevalence of exo A 33 (56.9%), exo S 21 (36.20%), exo U 37 (63.8%), and alg D 35 (60.34%) genes in ICU environment P. aeruginosa strains and exo A 23 (39.25%), exo S 25 (43.1%), exo U 40(68.98%), and alg D 25 (43.1%) genes in clinical isolates of P. aeruginosa. High resistance levels of the clinical and ICU environment isolate to ampicillin-sulbactam (100%), were observed too.

Conclusions: Our findings should raise awareness about antibiotic resistance in hospitalized patients in Iran. Clinicians should exercise caution in prescribing antibiotics, especially in cases of human infections.

Keywords:

Pseudomonas aeruginosa , Virulence Factors , Hospital infections

Affiliation:

Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan



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