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Evaluation of Various Diagnostic Techniques for the Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis at a Tertiary Care Center in North India


Monika Rajani* and Malay Banerjee   Pages 1 - 7 ( 7 )


Introduction: Tuberculosis is a major cause of mortality and morbidity worldwide. Bactec MGIT (Mycobacteria Growth Indicator tube) system is a rapid, reliable automated system for early diagnosis of pulmonary and extra pulmonary TB in setups where purchase of expensive instruments is not possible. The present study was thus carried out to evaluate AFB microscopy, culture on Lowenstein Jensen media and micro MGIT system for early, accurate and prompt diagnosis of Tuberculosis.

Methods: A total of 280 samples were processed for direct AFB smear examination, and culture on micro MGIT and LJ media. The identification of Mycobacterium tuberculosis complex in positive cultures was done by MPT64 Ag card test (BD MGIT TBC Identification Test).

Results: Out of the processed samples, 132/280(47.1%) were positive for Mycobacterium spp by Micro MGIT, 98/280(35%) on LJ medium and 72/280(25.7%) by AFB smear. A total of 136(48.5%) samples were positive by a combination of Micro MGIT and LJ medium. Among the total positive samples(136/280),Micro MGIT was found to be positive in 97%(132/136) of samples, LJ was positive in 72%(98/136),while 52.9%(72/136) were positive by AFB smear.

Conclusion: Manual MGIT System is a simple, efficient, safe to use diagnostic system. It does not require any expensive/special instrumentation other than the UV lamp for detection of fluorescence. In areas with limited resources where purchase of expensive instruments such as the MGIT 960 is out of scope, the use of manual MGIT for rapid susceptibility testing for MDR-TB could be an option.


Mycobacterium tuberculosis, micro MGIT, Lowenstein Jensen (LJ) media


Department of Microbiology, Career Post Graduate Institute of Medical Sciences and Hospital, Department of Microbiology, Career Post Graduate Institute of Medical Sciences and Hospital

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