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Molecular Characterization of Fasciola and Dicrocoelium Species Isolated from Ruminant Livestock in Qazvin, Iran

Author(s):

Mojtaba Shahnazi, Maryam Ebadi, Zahra Abbaspoor, Elham Hajialilo*, Amir Javadi, Peyman Heydarian, Mehrzad Saraei and Safar Ali Alizadeh   Pages 1 - 6 ( 6 )

Abstract:


INTRODUCTION: Fascioliasis and dicrocoeliasis are the most frequent zoonotic disease with increasing human health problems, in different parts of Iran. Two species of Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica) overlapping each other in the country. Molecular approaches have decisive place in identifying both species. The aim of this study was to detect Fasciola spp. and Dicrocoelium spp. by amplifying the ITS-2 and 28S rDNA gene sequence.

METHODOLOGY: Overall, 30 infected liver samples were collected from livestock of Qazvin, Iran. The adult flukes were collected from different livestock. DNA extraction and PCR amplification of ribosomal RNA gene region (ITS2) and 28S rDNA gene fragment were conducted and phylogenetic tree was constructed.

RESULT: All isolates obtained from cattle (No: 7) and 82.6% (No: 19) of sheep isolates were infected with F. hepatica species whereas 17.4% (No: 4) of sheep isolates were identified as F. gigantica. It was also shown that F. hepatica was the predominant species of Fasciola present in the region. All specimens were contaminated to Dicrocoelium dendriticum (D. dendriticum).

CONCLUSION: Both species of Fasciola were present in Qazvin. D. dendriticum was the sole infecting species of the Dicrocoelium genus in the livestock of the city of Qazvin. Further researches are needed to pinpoint the intermediate host of the parasites in the region.

Keywords:

Fasciola hepatica; Fasciola gigantica; Dicrocoelium dendriticum; Iran

Affiliation:

Cellular & Molecular Research Center, Research Institute for Prevention of Non- Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Cellular & Molecular Research Center, Research Institute for Prevention of Non- Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Department of Social Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin, Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin, Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin



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