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High-Level Aminoglycoside Resistance in Enterococcus Faecalis and Enterococcus Faecium; as a Serious Threat in Hospitals

Author(s):

Mahmoud Khodabandeh, Mohsen Mohammadi , Mohammad Reza Abdolsalehi, Meysam Hasannejad-Bibalan , Mehrdad Gholami , Azadeh Alvandimanesh, Abazar Pournajaf and Ramazan Rajabnia*   Pages 1 - 6 ( 6 )

Abstract:


The present work aimed to evaluate the frequency of aminoglycoside-modifying enzymes encoding genes in the E. faecalis and E. faecium and their antibiotic resistance profile. A total of 305 different clinical samples were subjected for identification and antibiotic susceptibility test. The high-level aminoglycoside resistance was identified by MIC and Kirby Bauer disc diffusion method. The prevalence of aac (6')-Ie-aph (2'')-Ia, aph (3')-IIIa and ant (4')-Ia genes was determined by multiplex- PCR. In total, 100 enterococci strains were isolated. The prevalence of E. faecalis and E. faecium isolates was 78% and 22%, respectively. All isolates were susceptible to linezolid. So, all E. faecalis were susceptible to vancomycin but, 36.4% of E. faecium were resistant to it. The prevalence of multiple drug resistance strains was 100% and 67.9% of E. faecium and E. faecalis, respectively. High-level-gentamicin and streptomycin resistant rates were as follows; 26.9% and 73.1% of E. faecalis and 77.3% and 90.1% of E. faecium. The results of the current study showed a high frequency of aac (6')-Ie-aph (2'')-Ia genes among enterococcal isolates. A high rate of resistance to antimicrobials in Enterococcus is obviously problematic, and a novel policy is needed to decrease resistance in these microorganisms.

Keywords:

Enterococcus, Aminoglycoside-modifying enzymes and Resistance.

Affiliation:

Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Molecular and Cell-Biology Research Center, Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Department of pathology, Shafa hospital, Qazvin University of medical sciences, Qazvin, Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol



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