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Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

[ Vol. 19 , Issue. 3 ]

Author(s):

Hassan Valadbeigi, Elham Esmaeeli, Sobhan Ghafourian, Abbas Maleki and Nourkhoda Sadeghifard*   Pages 322 - 326 ( 5 )

Abstract:


Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam.

Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes.

Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained.

Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

Keywords:

UPEC, gene profile, antibiotic susceptibility, urinary tract, molecular analysis, E.coli, molecular analysis.

Affiliation:

Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam



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