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Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

[ Vol. 19 , Issue. 3 ]


Saba Fakhrieh Asl, Mehrnaz Pourvahedi, Ali Mojtahedi * and Mohammad Shenagari   Pages 268 - 272 ( 5 )


Objective: Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endo-scopic patients in the north of Iran.

Methods: The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was ex-tracted from all isolated strains and PCR method was performed to detect the preva-lence of babA, cagE and cagA genes using specific primers.

Results: Among 90 samples of H. pylori, babA, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pat-tern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).

Conclusion: In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.


Helicobacter pylori, babA, cagE, cagA, gastric disorders, gastric patients.


Internal Medicine Department, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Genetics Department, Faculty of Basic Sciences, Guilan University, Rasht, Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Microbiology Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht

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