Arifuzzaman Khan*, Fahim Ashher, Fahmida Khanam, Mohammad Marufur Rahman, Momen Khan, Shyamal Kumar Paul and Mohammad Akram Hossain Pages 233 - 240 ( 8 )
Background: Widal test is the most widely used laboratory investigation for diagnosis of typhoid. However, the test interpretation remains controversial in the context of endemic regions such as Bangladesh, as agglutination occurs at varied titrations among a large percentage of healthy population. Paired Widal tests are often not feasible; hence single unpaired test has to be used for screening, diagnosis and treatment.
Objective: We aimed to assess the normal range of baseline titre for Anti TO, TH, AO, AH, BO agglutinins among healthy population in an endemic country with a view to guide the researchers and the clinicians, facilitating further investigation on updating cut off points of single Widal test for screening and diagnosis of typhoid fever in the context of Bangladesh.
Methods: A cross-sectional study was carried out in Mymensingh Medical College, Bangladesh on 2925 male immigration applicants. A single blood sample was collected for Widal test and interpreted using standard guidelines.
Results: The highest baseline titer for Anti TO, TH, AO, AH, BO agglutinins among 95% of the healthy participants was found to be 1:80 for each respectively. A titre of 1: 40 was observed for BH antigen.
Conclusion: In case of singular Widal test, baseline values for the normal range was found to be 1:20 - 1:80 for all the antigens (TO, TH, AO, AH, BO, BH), except BH, for which it was 1:20-1:40. Further studies, inclusive of other sociodemographic groups and positive controls are required to determine the updated cut off values.
Widal test, Typhoid fever, Paratyphoid fever, Screening test, Endemic regions, Artigens.
CSF Global, Dhaka, CSF Global, Dhaka, National Institute of Preventive and Social Medicine (NIPSOM), Dhaka, Center for Medical Biotechnology, MIS, DGHS, Department of Microbiology Mymensingh Medical College, Mymensingh, Department of Microbiology Mymensingh Medical College, Mymensingh, National Institute of Preventive and Social Medicine (NIPSOM), Dhaka