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Genetic Susceptibility in Family Members of Egyptian Hepatitis C Virus Infected Patients: Role of Interleukin-12 B Gene Polymorphism

[ Vol. 19 , Issue. 1 ]

Author(s):

Nadia Elwan, Fathia Assal, Asem Elfert, Lobna AboAli, Shaimaa Soliman, Samah Soliman, Walaa Elkhalawany, Rehab Badawy, Laila Effat, Khalda Sayed, Safinaz Shalaby, Marwa Shehab, Mohamed Abdel-Hamid and Sherief Abd-Elsalam*   Pages 81 - 87 ( 7 )

Abstract:


Aim: The research was conducted to study 1188 A\C polymorphism of Interleukin (IL)-12B gene for C/C, A/C and A/A genotypes in families of Hepatitis C virus (HCV) infected patients in Egypt.

Methods: Three hundreds HCV patients, 860 family members and 100 healthy subjects were studied. All family members were screened for HCV antibodies by enzyme-linked immunosorbent assay (ELISA). Positive cases were examined using Real-Time polymerase chain reaction (PCR) to confirm the presence of HCV ribonucleic acid (RNA) and detect the viral load. Molecular study of IL-12B gene was carried out on all patients, family members and controls using PCR and restriction enzyme analysis.

Results: HCV infection was confirmed in 10.6% of family members. The distribution of IL-12B gene polymorphism in patients was 2.3%, 45.7% and 52% for C/C, A/C and A/A genotypes respectively, in infected family members was 3.3%, 41.7%, 55%, in noninfected family members was 4.5%, 43.5% and 52% for C/C, A/C and A/A genotypes respectively and in control was 5%, 36% and 59% for C/C, A/C and A/A genotypes respectively. The frequency of the C/C, A/C and A/A genotype was not significantly different between the studied groups.

Conclusion: IL-12B gene polymorphism has no role in intrafamilial susceptibility of HCV transmission. The distribution of the functional 1188 A\C polymorphism of Interleukin (IL)-12B gene for C/C, A/C and A/A genotypes was not significantly different among the studied groups.

Keywords:

Gene, HCV, IL12B, intrafamilial transmission, polymorphism, genepolymorphism.

Affiliation:

Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Department of Public Health and Community Medicine, Menoufia University, Menoufia, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Medical Molecular Genetics and Cytogenetic, National Research Centre (NRC), El Behoosst, Dokki Cairo 11231, Departments of Medical Molecular Genetics and Cytogenetic, National Research Centre (NRC), El Behoosst, Dokki Cairo 11231, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta, Departments of Medical Molecular Genetics and Cytogenetic, National Research Centre (NRC), El Behoosst, Dokki Cairo 11231, Departments of Medical Molecular Genetics and Cytogenetic, National Research Centre (NRC), El Behoosst, Dokki Cairo 11231, Departments of Tropical Medicine and Public Health, Faculty of Medicine, Tanta University, El- Giash Street 31111, Tanta

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