Fatemeh Ahmadi-Motamayel, Samaneh Vaziri-Amjad, Mohammad Taghi Goodarzi and Jalal Poorolajal Pages 101 - 105 ( 5 )
Background: Saliva is a complex oral biologic fluid secreted by major and minor salivary glands. Saliva has immunological, enzymatic and antioxidant defense mechanisms. Infection with human immunodeficiency virus (HIV) is a life-threatening disease.Objective: The aim of this study was to evaluate salivary vitamin C and catalase levels in HIV-positive patients in comparison to a healthy control group. Method: Forty-nine HIV-infected individuals and 49 healthy subjects were selected. Five mL of unstimulated saliva was collected in 5 minutes using a sterilized Falcon tube with Navazesh method. Catalase and vitamin C levels were assessed by spectrophotometric assay. Data were analyzed with STATA 12. Results: Salivary catalase levels were 7.99±2.40 and 8.37±1.81 in the case and control groups, respectively. Catalase level was lower in the case group but the difference was not statistically significant (P=0.380). Salivary vitamin C levels in the case and control groups were 3.76±1.92 and 4.87±2.20, respectively (P=0.009). Conclusion: HIV can alter salivary antioxidant capacity as well as vitamin C and catalase levels. Saliva may reflect serum antioxidative changes in these patients. Therefore, further research is necessary on salivary and serum oxidants and the antioxidant changes.
Saliva, vitamin C, catalase, HIV positive, antioxidant, Spectrophotometric, saliva.
Dental Research Center and Research Center for Molecular Medicine, Department of Oral Medicine, Dental School, Hamadan University of Medical Sciences, Hamadan, Department of Oral Medicine, Hamadan University of Medical Sciences, Hamadan, Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Research Center for Health Sciences and Department of Epidemiology & Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan